Aqueous Extracts of the Edible <em>Gracilaria tenuistipitata</em> are Protective Against H<sub>2</sub>O<sub>2</sub>-Induced DNA Damage, Growth Inhibition, and Cell Cycle Arrest
Aqueous Extracts of the Edible <em>Gracilaria tenuistipitata</em> are Protective Against H<sub>2</sub>O<sub>2</sub>-Induced DNA Damage, Growth Inhibition, and Cell Cycle Arrest
Blog Article
Potential antioxidant properties of an aqueous extract of the edible red seaweed <em>Gracilaria tenuistipitata</em> (AEGT) against oxidative DNA damage were evaluated.The AEGT revealed several antioxidant molecules, including phenolics, flavonoids and ascorbic acid.In a cell-free assay, the extract exhibited 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity that significantly reduced H<sub>2</sub>O<sub>2</sub>-induced plasmid DNA breaks in a dose-response manner (<em>P</em> < 0.001).The AEGT also suppressed H<sub>2</sub>O<sub>2</sub>-induced oxidative Sports DNA damage in H1299 cells by reducing the percentage of damaged DNA in a dose-response manner (<em>P</em> < 0.
001) as measured by a modified alkaline comet-nuclear extract (comet-NE) assay.The MTT assay results showed that AEGT confers significant protection against H<sub>2</sub>O<sub>2</sub>-induced cytotoxicity Tracksuit and that AEGT itself is not cytotoxic (<em>P</em> < 0.001).Moreover, H<sub>2</sub>O<sub>2</sub>-induced cell cycle G2/M arrest was significantly released when cells were co-treated with different concentrations of AEGT (<em>P</em> < 0.001).
Taken together, these findings suggest that edible red algae <em>Gracilaria</em> water extract can prevent H<sub>2</sub>O<sub>2</sub>-induced oxidative DNA damage and its related cellular responses.